Journal: Applied and Environmental Microbiology
Article Title: Plasmid-free cheater cells commonly evolve during laboratory growth
doi: 10.1128/aem.02311-23
Figure Lengend Snippet: V. cholerae ΔvpsL with the plasmid c-di-GMP biosensor (pRP0122) was propagated on a gradient of increasing concentrations of either ampicillin or carbenicillin. A representative colony is shown, and the inlet provides a closer look at the edge of the colony.
Article Snippet: Ampicillin [D-(-)-α-Aminobenzylpenicillinsodium salt, Gold Biotechnology], carbenicillin (Carbenicillin (disodium), Gold Biotechnology), and kanamycin (Kanamycin monosulfate, Gold Biotechnology) were prepared at a concentration of 100 mg/mL by dissolving 1 g of antibiotic in 10 mL of deionized water.
Techniques: Plasmid Preparation